安徽省域耕地等别变化调查与质量建设对策

该文总结了安徽省域耕地等别分布现状、与上轮成果对比分析,分析了规律与原因,进而提出了定期更新耕地质量分等成果、完善耕地质量监管机制等方面的耕地质量建设对策,以期未来的耕地质量建设与管理更利于"双保"战略的落实。     

水利施工的大体积混凝土抗裂技术

混凝土是水利施工中的主要的应用材料之一，为了保证水利施工中的安全与质量，必须保证混凝土的质量。文章全面分析了大体积混凝土产生裂缝的原因，并给出了相应的控制方法。     

有效体育教学模式的创建与实施*

有效体育教学的内涵主要包括三个方面，即，体育教学要重效果，体育教学要好效益，体育教学要高效率。有效体育教学模式是指在“健康第一”思想指导下相对稳定的一种教学程序，其创建步骤主要包括确立教学指导思想、明晰目标内涵、分析教学情境、编选教学内容、创设运作程式。有效体育教学模式的实施要全程渗透健康引领的价值观念，细化体育教学有效性的指标体系，加强教师的有效指导，激活学生的主体参与。     

水利工程建设生态风险评估模型探析

本文针对我国水能资源理性有序开发问题,采用专家组评分与本底资料查阅等方式,探索构建水利工程建设生态风险评估模型.该模型可定量与定性分析水利工程建设中对生态系统产生的危害与事故发生的概率,模型对水能资源开发生态评估具有较强适用性.本研究可对水利工程建设与水能资源开发决策提供有益借鉴.     

Screening of lentiviral vectors carrying effective siRNA targeting S1P2 gene

AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×10⁵ cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency.     

园林喷灌工程的施工方法研究

对园林喷灌施工技术的强化，是保护绿地康健进步的有效途径和关键措施，同时，是促进我国资源节约形社会建设的客观要求，在实践的过程中，遵守喷灌技术是设计方的基本原则，为我国高速发展的园林绿地建设服务。     

广东新农村建设的现状分析与发展对策

针对广东新农村建设存在的规划滞后、资源浪费、污染严重、社会管理水平低、环保意识薄弱等问题，提出用生态文明理念引领新农村建设以及建设广东生态文明新农村的对策。     

我国省级农业科学院的学科竞争力比较研究*

学科竞争力是省级农业科研机构科研实力的重要体现。文章以江浙沪鲁京粤6省农业科学院为研究对象，以SCI论文和中文核心期刊论文为数据源，采用文献计量的方法，对比分析了我国省级农科院的整体优势学科和各自优势学科的竞争力情况。研究发现，省级农科院的学科发展既有普遍相似性，又有各自的特色优势学科，各机构之间的学科竞争力差距较大。该研究为我国省级农科院学科建设提供决策参考。     

加强农业科研院所廉政建设的几点思考

近年来，随着中央对“三农”工作的重视，投入的农业科研项目资金越来越大，这对农业科研院所的壮大起到了强有力的推动作用，但随之而来的贪腐行为时有发生，并呈上升趋势。文章就当前农业科研院所廉政建设工作现状及面临的问题，进行了深入分析，并提出了进一步加强廉政建设的工作对策，从而更好地构建廉政建设体系，保证农业科研院所事业又好又快发展。     

Effect Comparison of Adenovirus Vector Transfer into Different Buffalo Cells

Buffalo mammary epithelial cell,cumulus cell and fibroblasts were transfected by adenovirus vectors and compared their transfection efficiency.293 cells were transfected with pBHGloxdelE13cre and pDC316-eGFP by liposome,the virus was collected and titer was detected.Buffalo mammary epithelial cell,cumulus cell and fibroblasts were exposed to different multiplicity of infection (MOI) of adenovirus vectors.After 72 h,the cells were observed with inverted fluorescence microscope,and transfection efficiency was calculated.When the MOI was 25,50,100,200 and 400,the transfection efficiency of fibroblasts were 0.7%,7.0%,9.0%,12.5% and 34.0%,the transfection efficiency of cumulus cells were 42.5%,55.3%,57.4%,76.0% and 80.0%,the transfection efficiency of mammary epithelial cells were 88.7%,100%,100%,100% and 100%.The results showed that the transfection efficiency of mammary epithelial cell was the best,followed by cumulus cell,and fibroblast was poor.